Method for detecting content of lactic acid and glycollic acid in microsphere agent for injection

The invention discloses a method for detecting content of lactic acid and glycollic acid in a microsphere agent for injection. The method comprises the following steps: (1) preparing a to-be-detected microsphere sample solution for injection, namely precisely weighing 50mg of a sample for test, addi...

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Hauptverfasser: CHEN JIAOYAN, YANG JINGJIN, DU BAOGUO, DING DUOHAO, FEI YAN, ZHAO GENHUA, ZHOU SANYUN, HU TIANYU, MIAO ZHENHUA, XIA DIANRONG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a method for detecting content of lactic acid and glycollic acid in a microsphere agent for injection. The method comprises the following steps: (1) preparing a to-be-detected microsphere sample solution for injection, namely precisely weighing 50mg of a sample for test, adding the sample into a 2mL of centrifuge tube, adding 0.5mL of dichloromethane, whirling to dissolve for 1 minute, adding 0.5mL of water for performing vortex extraction for 1 minute, centrifuging in a centrifugal machine, taking out the centrifuge tube, and taking the upper aqueous liquid as the to-be-detected sample solution; and (2) performing high performance liquid chromatography, namely respectively taking the to-be-detected sample solution and a reference solution, injecting into a liquid chromatograph, measuring and recording chromatogram according to the set detection conditions, and calculating the content of the content of lactic acid and glycollic acid in the to-be-detected sample according to an external