Optimized target object capturing system based on bacterial cell surface display system
The invention discloses an optimized target object capturing system based on a bacterial cell surface display system, namely, the optimized target object capturing system comprises the following steps: inserting a segment of coding genes of adaptor protein between a virus capsid protein coding gene...
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| creator | XU QIAN NI PEI'EN YIN YUJIE WANG DAPENG LIU DANLEI SHI XIANMING |
| description | The invention discloses an optimized target object capturing system based on a bacterial cell surface display system, namely, the optimized target object capturing system comprises the following steps: inserting a segment of coding genes of adaptor protein between a virus capsid protein coding gene and a coding gene inaQn which is responsible for transmembrane, transfer and anchoring and is in an N-terminal structure domain of ice-nucleation protein, and establishing recombinant plasmid of inducible surface display virus capsid protein after verifying; transforming host bacteria, thus obtaining an updated bacterial cell surface display system of capsid protein; displaying virus capsid protein on the surface of escherichia coli cells through inducible expression, thus obtaining an optimized virus receptor capturing system. Compared with a traditional virus receptor purification and analysis method, the bacterial cell surface display system has the advantages that the cost of a reagent is low, the operation is |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Optimized target object capturing system based on bacterial cell surface display system |
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