Optimized target object capturing system based on bacterial cell surface display system

The invention discloses an optimized target object capturing system based on a bacterial cell surface display system, namely, the optimized target object capturing system comprises the following steps: inserting a segment of coding genes of adaptor protein between a virus capsid protein coding gene...

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Hauptverfasser: XU QIAN, NI PEI'EN, YIN YUJIE, WANG DAPENG, LIU DANLEI, SHI XIANMING
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses an optimized target object capturing system based on a bacterial cell surface display system, namely, the optimized target object capturing system comprises the following steps: inserting a segment of coding genes of adaptor protein between a virus capsid protein coding gene and a coding gene inaQn which is responsible for transmembrane, transfer and anchoring and is in an N-terminal structure domain of ice-nucleation protein, and establishing recombinant plasmid of inducible surface display virus capsid protein after verifying; transforming host bacteria, thus obtaining an updated bacterial cell surface display system of capsid protein; displaying virus capsid protein on the surface of escherichia coli cells through inducible expression, thus obtaining an optimized virus receptor capturing system. Compared with a traditional virus receptor purification and analysis method, the bacterial cell surface display system has the advantages that the cost of a reagent is low, the operation is