Polyclonal antibody of bovine transmembrane epididymis protein 1, and preparation method thereof

The invention belongs to the technical field of gene engineering, and concretely discloses a polyclonal antibody of bovine transmembrane epididymis protein 1, and a preparation method thereof. The polyclonal antibody is prepared through an animal immunization technology by adopting artificially synthesized polypeptide as an antigen; and the amino acid sequence of the artificially synthesized polypeptide is represented by SEQ ID NO.1. The prepared polyclonal antibody reacts with the antigen of the bovine transmembrane epididymis protein 1, and lays a foundation for the studies of the expression of the bovine transmembrane epididymis protein 1. 本发明属于基因工程技术领域，具体公开了种牛跨膜附睾蛋白1的多克隆抗体及制备方法，所述多克隆抗体以人工合成多肽为抗原，采用动物免疫方法制备而成；所述人工合成多肽的氨基酸序列如SEQ ID NO.1所示。本发明制备的多克隆抗体能够与牛跨膜附睾蛋白1的抗原发生作用，为牛跨膜附睾蛋白1的表达研究工作奠定基础。