Method for rapidly breeding wild bulb of fritillaria unibracteata

The invention discloses a method for rapidly breeding a wild bulb of fritillaria unibracteata. The method comprises the following steps: obtaining a sterile squamule; inducing a callus tissue and a cluster bud of the sterile squamule; conducting propagation culture on the cluster bulblet; inducing rooting of the regenerated bulblet; transplanting and domesticating the rooted bulblet. By adopting the technical scheme provided by the invention, the breeding rate of the fritillaria unibracteata is increased by compared with the previous researches, and the problems of easiness in browning, difficulty in rooting, more abnormal roots and the like in the breeding process of the regenerated bulblet of the fritillaria unibracteata are solved. 本发明公开了种暗紫贝母野生鳞茎快速繁殖的方法，该方法包括以下步骤：无菌小鳞片的获得；无菌小鳞片愈伤组织和丛生芽的诱导；丛生小鳞茎的增殖培养；再生小鳞茎的生根诱导；生根小鳞茎的移栽驯化。采用本发明的技术方案，暗紫贝母增殖率较前人研究有所提高；解决了暗紫贝母再生小鳞茎增殖过程中易褐化、生根难、畸形根多等问题。