Preparation method and using method of human mesenchymal stem cell exosome freeze-dried powder

The invention provides a preparation method and a using method of human mesenchymal stem cell exosome freeze-dried powder, used for solving the problems of exosome such as low separation and recovery quantity, poor quality and low efficiency. The preparation method of the human mesenchymal stem cell exosome freeze-dried powder comprises the following steps: 1) performing culture, purification and passage on human mesenchymal stem cells; 2) inducing large-scale synthesis of the human mesenchymal stem cells and secreting functional exosome; 3) performing ultrafiltration and concentration on the exosome of the human mesenchymal stem cells, preparing a concentrated culture solution, and refrigerating; 4) preparing the freeze-dried powder from the refrigerated concentrated culture solution, and adding complex liquid into the freeze-dried powder for preserving. According to the method disclosed by the invention, synthesis of the human mesenchymal stem cells and secretion of the exosome can be effectively induced to