Screening and application of high-resistance trehalose-producing saccharomyces cerevisiae strain
The invention provides screening and application of a high-resistance trehalose-producing saccharomyces cerevisiae strain. A preservation number of the strain is CCTCC (China Center for Type Culture Collection) NO: M 2012473. The screening comprises the steps of collecting spontaneously fermented Ci...
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Zusammenfassung: | The invention provides screening and application of a high-resistance trehalose-producing saccharomyces cerevisiae strain. A preservation number of the strain is CCTCC (China Center for Type Culture Collection) NO: M 2012473. The screening comprises the steps of collecting spontaneously fermented Citrus verrucusa Hort. juice, sucking 0.2mL, evenly coating on a culture dish where sterilized bean sprout juice agar culture medium is contained, culturing in a 28 DEG C constant-temperature box for 2d and then performing microscopic examination; picking a strain with good growing bacterial colony, obvious saccharomycetes colony characteristics and light fruit fragrance; on a sterile operation bench, picking the chosen saccharomycetes to be streak inoculated on a fresh slop culture medium and culturing for 3d; then sequentially performing partition streak culture until a pure single strain is separated out. The trehalose saccharomyces cerevisiae strain disclosed by the invention can be used for fermenting and produc |
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