SNP (Single Nucleotide Polymorphism) and detection kit for early evaluation of esophageal squamous carcinoma onset risk

The invention discloses a detection kit for single nucleotide polymorphism sites in high correlation to esophageal squamous carcinoma incidence. The kit comprises two forward allele specific primers aiming at the 5-lipoxygenase E254K site design, one reverse primer, an internal reference primer, PCR (Polymerase Chain Reaction) mix and sterile water. The kit adopted by the invention is capable of rapidly detecting 5-LO E254K sites in high correlation to esophageal squamous carcinoma incidence, and has the characteristics of simplicity, economic efficiency, rapidness and the like compared with the classical sequencing and enzyme digestion methods, and an allele specific PCR method based on the kit disclosed by the invention is a method which is mature and reliable and can be widely used in primary hospitals. 本发明是个与食管鳞癌发生高度相关的单核苷酸多态性位点的检测试剂盒。该试剂盒包括针对5-脂氧合酶E254K位点设计的上游等位基因特异性引物2条，下游引物1条，对内参照引物，PCR mix，灭菌水。本发明所采用的试剂盒能够快速检测出与食管鳞癌发生高度相关的5-LO E254K位点，与经典的测序法和酶切法相比具有简便、经济、快速等特点，并且本发明所基于的等位基因特异性PCR法是种成熟可靠、可在基层医院广泛使用的方法