PK-15 cell culture fluid, and preparation method and application method thereof

The invention discloses a PK-15 cell culture fluid, and a preparation method and an application method thereof and belongs to the technical field of veterinary biological products. The cell culture fluid comprises the following components: DMEM powder, astragalus polysaccharide, puerarin, atractylenolide I, L-Asp, L-Lys, an insulin-like growth factor-1, sodium pyruvate, penicillin and streptomycin. The used PK-15 cell culture fluid has the beneficial effects that the dosage of serum is greatly reduced, the cost of cell culture is reduced, the growth rate of a PK-15 cell is improved, the sensitivity of PCV-2 on the PK-15 cell is strengthened and the content of a virus is improved. Due to the used PK-15 cell culture fluid, the immunogenicity of a PCV-2 vaccine can be greatly improved. 种PK-15细胞培养液及其制备方法、使用方法，属于兽用生物制品技术领域。该细胞培养液每1000ml去离子水中含有以下组分：DMEM粉末、黄芪多糖、葛根素、白术内酯Ⅰ、L-Asp、L-Lys、胰岛素样生长因子-I、丙酮酸钠、青霉素和链霉素。本发明具有以下有益效果：本发明所使用的PK-15细胞培养液，大大减少了血清的使用量，降低了细胞培养的成本；提高PK-15细胞生长速度；增强PCV-2对PK-15细胞的敏感性，提高了病毒含量；由本发明所使用的PK-15细胞培