Marc-145 cell culture liquid, and preparation method and use method thereof

The invention provides a Marc-145 cell culture liquid, and a preparation method and a use method thereof, and belongs to the technical field of biological products for veterinary use. In the cell culture liquid, per 1000 ml of deionized water contains the following components: a DMEM powder, insulin-like growth factors, zinc glycinate, sodium selenite, sodium bicarbonate, transferring, ginsenoside and epimedium polysaccharide. The Marc-145 cell culture liquid, and the preparation method and the use method thereof have the following beneficial effects: the Marc-145 cell culture liquid greatly reduces the use amount of serum and reduces the cell culture cost; the growth speed of the Marc-145 cells is increased; the sensitivity of PRRSV (porcine reproductive and respiratory syndrome) on the Marc-145 cells is enhanced and the virus content is increased; the Marc-145 cell culture liquid can greatly improve the immunogenicity of PRRSV vaccines. 种Marc-145细胞培养液及其制备方法、使用方法，属于兽用生物制品技术领域。该细胞培养液每1000ml去离子水中含有以下组分：DMEM粉末、