Method for observing three-dimensional structure of trachea of insect by micro CT (Computed Tomography)

The invention provides a method for observing the three-dimensional structure of the trachea of an insect by micro CT (Computed Tomography). The method comprises the following steps: a, sample treatment: performing tissue fixation on a respiratory organ of a target insect by using a fixing solution at the temperature of 4 DEG C; b, micro CT observation: observing a treated sample by micro CT. The method has the advantages of observing the three-dimensional characteristic of the respiratory organ, rebuilding and observing the structure of any plane, along with no tissue damage, high speed, high efficiency and high repeatability. By adopting the method, the basic structure of the insect can be known comprehensively from outside to inside and from a plane to three dimension, and an important basis and a technical measure are provided for comprehensive observation of the internal respiratory system structure of the insect. 本发明提供了种采用显微CT观察昆虫气管立体结构的方法，包括以下步骤：a、样本处理，在温度为4℃的条件下使用固定液将目标昆虫的呼吸器官进行组织固定；b、显微CT观察，利用显微CT对处理