Novel lysine decarboxylase and application thereof

The invention discloses novel lysine decarboxylase. The amino acid sequence of the novel lysine decarboxylase is shown as SEQ ID NO: 1 or SEQ ID NO: 2. The lysine decarboxylase can be expressed efficiently in a host cell, has superior thermal stability, and has the characteristics of high activity under a neutral to weakly-alkaline condition, so that the novel lysine decarboxylase is superior to conventional lysine decarboxylase. The invention further provides an expression vector including an encoding sequence of the lysine decarboxylase, a host cell capable of expressing proteins, application of the expression vector and the host cell to production of 1,5-pentamethylene diamine, and a production method. 本发明公开了新的赖氨酸脱羧酶，其氨基酸序列如SEQ ID NO:1或2所示。本发明的赖氨酸脱羧酶能够在宿主细胞中高效表达且具备优异的热稳定性、在中偏碱性条件下具有高活力等特性，从而优于现有的赖氨酸脱羧酶。本发明还提供了包含所述赖氨酸脱羧酶的编码序列的表达载体、包含能够表达所述蛋白的宿主细胞以及它们在产生1,5-戊二胺中的用途和生产方法。