Novel lysine decarboxylase mutant and application thereof

The invention discloses a novel lysine decarboxylase, the amino acid sequence of which is from the mutation of a sequence shown by SEQ ID NO:1, and one or more amino acid residue sites chosen from the following group are mutated: site 9, site 44, site 88, site 111, site 176 and site 230. The activity of 1,5-pentanediamine produced from lysine catalyzed by lysine decarboxylase disclosed by the invention is remarkably increased, consequently, the dosage of a catalyst can be reduced, and ultimately the production cost is reduced. The invention further provides an expression vector containing a coded sequence of the lysine decarboxylase, a host cell capable of expressing the lysine decarboxylase, application of the expression vector and the host cell in the production of the 1,5-diaminopentane and production methods. 本发明公开了新的赖氨酸脱羧酶，其氨基酸序列由SEQ ID NO:1所示序列突变而来，在选自下组的个或多个氨基酸残基位点发生突变：9位、44位、88位、111位、176位和230位。本发明的赖氨酸脱羧酶催化赖氨酸产生1,5-戊二胺的活性显著提高，从而可以降低催化剂的用量最终降低生产成本。本发明还提供了包含所述赖氨酸脱羧酶的编码序列的表达载体、包含能够表达所述赖氨酸脱羧酶的宿主细胞以及它们在产生1,