DF-1 cell culture fluid additive as well as preparation method and use method thereof

The invention discloses a DF-1 cell culture fluid additive as well as a preparation method and a use method thereof, and belongs to the technical field of veterinary biological products. Every 1000ml PBS (Phosphate Buffered Solution) of cell culture fluid comprises the following components: 10-30mg of hedysarum polybotys saccharide, 5-15mg of naringenin, 5-15mg of astragaloside, 10-20mg of a cuscuta chinensis extract, 1-10mg of putrescine and 5-20mg of glutathione. The DF-1 cell culture fluid additive disclosed by the invention has the beneficial effects that by using the DF-1 cell culture fluid additive, the use amount of serum is greatly reduced, the cost of cell culture is lowered, the growth speed of DF-1 cells is increased, the sensitivity of the IBDV (Infectious Brusal Disease Virus) to DF-1 cells is improved, and the content of TCID50 is increased; by adopting the DF-1 cell culture fluid, the immunogenicity of chicken infectious bursal disease live vaccines can be greatly improved. 种DF-1细胞培养液添加剂及其制备方法、