Primer set for sex determination in turbot and method for sexdetermination in turbot using these primers

The present invention provides a primer set for sex determination in turbots, which is characterized by containing a primer SEQ ID No:1 (5'-TGAGAAAGAGCTGCTGGTGA-3'), or a SEQ ID No:2 (5'-CGTCTTGTGCTTCTGGTGAA-3') of aromatase genes which are expressed specifically in the reproductive organs of female turbots. For an accurate comparison of the aromatase expression, the present invention also provides primer set SEQ ID No:3 (5'-ACCGTCAGACCCTGATGTTC-3') and SEQ ID No:4 (5'-GCGTTCTGTTCCTGTGGTTT-3') of vasa genes specific to the reproductive organs of the turbot, and primer set SEQ ID No:5 (5'-CGTTGGTGTCAACAAGATGG-3') and SEQ ID No:6 (5'-ATCCAGAGATGGGGACAAAG-3') of house-keeping ef1a gene to determine the sex of the turbot at the breeding stage, and thereby increase the farming efficiency of turbots which have recently become a promising aquaculture species. 本发明提供用于大菱鲆雌雄辨别的引物组，其特征在于，包含在大菱鲆的雌性生殖质中特异性地表达的芳香化酶基因的引物组SEQ ID No:1(5'-TGAGAAAGAGCTGCTGGTGA-3')、SEQ ID No:2(5'-CGTCTTGTGCTTCTGGTGAA-3')。另外，为了准确地比较芳香化酶的表达，提供大菱鲆生