PEG4000 mediated potato protoplast transformation method and application thereof
The invention relates to the field of cell engineering, in particular to a PEG4000 mediated potato protoplast transformation method and application thereof. The method includes: well mixing prepared potato protoplast with PEG4000 solution being 40v/v% in concentration and vector plasmids connected w...
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| creator | HAN ZHONGKUI LIU JUN XIE CONGHUA CAI XINGKUI |
| description | The invention relates to the field of cell engineering, in particular to a PEG4000 mediated potato protoplast transformation method and application thereof. The method includes: well mixing prepared potato protoplast with PEG4000 solution being 40v/v% in concentration and vector plasmids connected with target genes, treating in dark at the room temperature for 5-7min, removing supernatant, and subjecting precipitates to resuspension culture to obtain a transformant. The method is convenient and simple in operation, low in cost and low in harms to protoplasts, and the transformation rate reaches about 70%.
本发明涉及细胞工程领域，具体而言，涉及种PEG介导的马铃薯原生质体转化方法及其应用。该方法包括：将制备好的马铃薯原生质体与40v/v％浓度的PEG溶液、连接有目的基因的载体质粒混匀，在室温下黑暗处理5～7min后弃去上清并将沉淀重悬培养即得转化子。本方法操作简便，成本相对较低，转化率可达70％左右，且对原生质体伤害较小。 |
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本发明涉及细胞工程领域，具体而言，涉及种PEG介导的马铃薯原生质体转化方法及其应用。该方法包括：将制备好的马铃薯原生质体与40v/v％浓度的PEG溶液、连接有目的基因的载体质粒混匀，在室温下黑暗处理5～7min后弃去上清并将沉淀重悬培养即得转化子。本方法操作简便，成本相对较低，转化率可达70％左右，且对原生质体伤害较小。</description><language>chi ; eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; NEW PLANTS OR PROCESSES FOR OBTAINING THEM ; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TRAPPING ; VINEGAR ; WINE</subject><creationdate>2017</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20170829&DB=EPODOC&CC=CN&NR=107099552A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20170829&DB=EPODOC&CC=CN&NR=107099552A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>HAN ZHONGKUI</creatorcontrib><creatorcontrib>LIU JUN</creatorcontrib><creatorcontrib>XIE CONGHUA</creatorcontrib><creatorcontrib>CAI XINGKUI</creatorcontrib><title>PEG4000 mediated potato protoplast transformation method and application thereof</title><description>The invention relates to the field of cell engineering, in particular to a PEG4000 mediated potato protoplast transformation method and application thereof. The method includes: well mixing prepared potato protoplast with PEG4000 solution being 40v/v% in concentration and vector plasmids connected with target genes, treating in dark at the room temperature for 5-7min, removing supernatant, and subjecting precipitates to resuspension culture to obtain a transformant. The method is convenient and simple in operation, low in cost and low in harms to protoplasts, and the transformation rate reaches about 70%.
本发明涉及细胞工程领域，具体而言，涉及种PEG介导的马铃薯原生质体转化方法及其应用。该方法包括：将制备好的马铃薯原生质体与40v/v％浓度的PEG溶液、连接有目的基因的载体质粒混匀，在室温下黑暗处理5～7min后弃去上清并将沉淀重悬培养即得转化子。本方法操作简便，成本相对较低，转化率可达70％左右，且对原生质体伤害较小。</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</subject><subject>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TRAPPING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2017</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNiksKwjAQQLNxIdU7xAMI46dIl1KqrqQL92VoJjSQZoZk7k8LegAXjwePtzV93z2vAGBncgGVnBVWVLaSWVkiFrWaMRXPeUYNnNZTJ3YW04pIDOM360SZ2O_MxmMstP-5ModH92lfRxIeqAiOlEiH9n2CGzRNXZ_vl3-eBV8SNzs</recordid><startdate>20170829</startdate><enddate>20170829</enddate><creator>HAN ZHONGKUI</creator><creator>LIU JUN</creator><creator>XIE CONGHUA</creator><creator>CAI XINGKUI</creator><scope>EVB</scope></search><sort><creationdate>20170829</creationdate><title>PEG4000 mediated potato protoplast transformation method and application thereof</title><author>HAN ZHONGKUI ; LIU JUN ; XIE CONGHUA ; CAI XINGKUI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN107099552A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2017</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</topic><topic>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TRAPPING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>HAN ZHONGKUI</creatorcontrib><creatorcontrib>LIU JUN</creatorcontrib><creatorcontrib>XIE CONGHUA</creatorcontrib><creatorcontrib>CAI XINGKUI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>HAN ZHONGKUI</au><au>LIU JUN</au><au>XIE CONGHUA</au><au>CAI XINGKUI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>PEG4000 mediated potato protoplast transformation method and application thereof</title><date>2017-08-29</date><risdate>2017</risdate><abstract>The invention relates to the field of cell engineering, in particular to a PEG4000 mediated potato protoplast transformation method and application thereof. The method includes: well mixing prepared potato protoplast with PEG4000 solution being 40v/v% in concentration and vector plasmids connected with target genes, treating in dark at the room temperature for 5-7min, removing supernatant, and subjecting precipitates to resuspension culture to obtain a transformant. The method is convenient and simple in operation, low in cost and low in harms to protoplasts, and the transformation rate reaches about 70%.
本发明涉及细胞工程领域，具体而言，涉及种PEG介导的马铃薯原生质体转化方法及其应用。该方法包括：将制备好的马铃薯原生质体与40v/v％浓度的PEG溶液、连接有目的基因的载体质粒混匀，在室温下黑暗处理5～7min后弃去上清并将沉淀重悬培养即得转化子。本方法操作简便，成本相对较低，转化率可达70％左右，且对原生质体伤害较小。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE |
| title | PEG4000 mediated potato protoplast transformation method and application thereof |
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