Kit and detection method for detecting mycobacterium tuberculosis infection

The invention discloses a kit for detecting mycobacterium tuberculosis infection. The kit comprises the following components: a detection primer, a 10-fold loop-mediated isothermal amplification reaction liquid, a DNA polymerase, a positive control, a negative control, a color developing agent and a bacteria spreading cultivation liquid, wherein the detection primer comprises a pair of outer primers F3/B3 and a pair of inner primers FIP/BIP. The kit is capable of efficiently, quickly, accurately and sensitively obtaining the detection result of mycobacterium tuberculosis infection in blood, the detection result is high in specificity and high in sensitivity, result judgment can be easily completed with eyes instead of electrophoresis, and the kit is very suitable for fast detection of mycobacterium tuberculosis. 本发明公开了种用于结核分枝杆菌感染检测的试剂盒，包括如下部分：由检测引物、10倍环介导等温扩增反应液、DNA聚合酶、阳性对照、阴性对照、显色剂和菌体扩培液组成；所述的检测引物是由对外引物F3/B3和对内引物FIP/BIP组成。该试剂盒能够高效、快速、准确、灵敏的得到血液中结核分枝杆菌感染的检测结果，且检测结果特异性高、敏感性强，结果判断不需要使用电泳、眼易判断，非常适用于结核分枝杆菌的快速检测。