Unfertilized ovary in-vitro culture method of Allium tuberosum

The invention belongs to haploid culture techniques and provides an unfertilized ovary in-vitro culture method of Allium tuberosum; unfertilized ovary of Allium tuberosum is used herein as a subject, ovary pretreatment, inoculation, transferring and rooting steps are performed, and haploid plants of Allium tuberosum are finally acquired; the specific steps include (1) pretreating the ovary; (2) inoculating; (3) transferring; (4) rooting. Compared with the prior art, the unfertilized ovary in-vitro culture method of Allium tuberosum has relatively shortened culture time; both optimized culture media and culture method give rise to the great increase in production count of ovule and production rate of ovule; the optimized rooting media impart more effective rooting and higher survival rate to obtained plants; under the cooperation of the methods, breeding time is shortened. 本发明属于单倍体培养技术，提供种韭菜未受精子房离体培养方法，该方法以韭菜未受精子房作为对象，经过子房预处理、接种、转接、生根步骤，最终获得韭菜单倍植株，具体步骤如下：(1)子房预处理(2)接种(3)转接(4)生根。该方法相对于传统方法，培养时间相对缩短，通过优化的培养基和培养方