MDBK cell culture liquid, and preparation method and use method thereof

The invention discloses an MDBK cell culture liquid, and a preparation method and a use method thereof, and belongs to the technical field of veterinary biological products. The cell culture liquid is characterized in that every 1000 ml of deionized water contains DMEM powder, a transferrin stock solution, a bFGF stock solution, an EGF stock solution, IL-3, propolis flavones, Radix Astragali saponins, penicillin and streptomycin. The MDBK cell culture liquid has the advantages of great reduction of the use amount of serum, reduction of the cell culture cost, increase of the growth speed of MDBK cells, enhancement of the sensitivity of BVDV to the MDBK cells, increase of the virus content, and great improvement of the immunogenicity of BVD-MD inactivated vaccines. 种MDBK细胞培养液及其制备方法、使用方法，属于兽用生物制品技术领域。该细胞培养液每1000ml 去离子水中含有以下组分：DMEM粉末、转铁蛋白储液、bFGF储液、EGF储液、IL-3、蜂胶黄酮、黄芪皂苷、青霉素、链霉素。本发明具有以下有益效果：本发明所使用的MDBK细胞培养液，大大减少了血清的使用量，降低了细胞培养的成本；提高MDBK细胞生长速度；增强BVDV对MDBK细胞的敏感性，提高了病毒含量；由本发明所使用的MDBK细胞培养液还可以大幅度提高BVD-MD灭活疫苗的免疫原性。