System for efficient screening of candidate sgRNAs for CRISPR/CAS9 gene editing system from plants and application

The invention discloses a system for high-throughput screening of candidate sgRNA target sites for a CRISPR/CAS9 system and application thereof. The testing system comprises 1, plasmids for expressing multiple candidate sgRNAs at the same time; and 2, plasmids for expressing CAS9. Fast high-throughput identification of the cutting efficiency of the candidate sgRNA target sites can be achieved by means of an arabidopsis and rice protoplast transient expression system. It is important to select a target sequence capable of achieving efficient cutting before the CRISPR/CAS9 system knocks out or edits genes, the cutting efficiency of multiple candidate sgRNA target sites can be verified within a short period of time, so that the optimal sgRNA can be selected to improve the success rate of knockout, in this way, the working cost can be reduced, and working efficiency can be improved. 本发明公开了种CRISPR/CAS9系统候选sgRNA靶位点高通量筛选系统及其应用，所述测试系统包括：(1)用于同时表达多个候选sgRNA的质粒；(2)用于表达CAS9的质粒；(3)利用拟南芥及水稻原生质体瞬时表达系统快速高通量鉴定候选sgRNA靶位点切割效率。C