PCR (polymerase chain reaction) system and kit for high-GC segment amplification
The invention belongs to the technical field of biology, and particularly relates to the technical field of PCR (polymerase chain reaction), more particular to a PCR (polymerase chain reaction) system and kit for high-GC segment amplification. The PCR system and kit comprises dNTPs, Tris-HCl, MgSO4,...
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| creator | PENG CHUNMEI MO JINGYAN CHEN GUANZHI CHEN FENGYING WANG FA ZHANG XIN LI HAIYIN LIN MINSHEN WANG XING YU PEIYU YUE XIAOYAN XIE LIJUAN ZHANG XIAOWEI LI JIADAO LIN ZHIHAO ZHANG JIA LIN RUOLIN SHI ZHUANGZHUANG LUO YUANXIANG DENG KEJI |
| description | The invention belongs to the technical field of biology, and particularly relates to the technical field of PCR (polymerase chain reaction), more particular to a PCR (polymerase chain reaction) system and kit for high-GC segment amplification. The PCR system and kit comprises dNTPs, Tris-HCl, MgSO4, (NH4)2SO4 and PCR auxiliary, wherein the PCR auxiliary is one or multiple of BeCl2, dimethyl sulfoxide (DMSO), betaine and dithiothreitol (DTT). Compared with methods for amplifying high segments through 7-deaza-dGTP, the PCR system and kit has the advantage that cost is lowered greatly. Compared with existing high-GC-content amplification methods, the PCR system can amplify DNA segments higher in GC content, can amplify DNA segments higher than 80% in GC content and even can amplify DNA segments up to 90% in GC content.
本发明属于生物技术领域，具体涉及PCR技术领域，更具体涉及种用于高GC片段扩增的PCR反应体系及试剂盒，所述的PCR反应体系和试剂盒包括：dNTPs、Tris-HCl、MgSO、(NH)SO和PCR助剂。所述的PCR助剂为BeCl、二甲基亚砜(DMSO)、甜菜碱和二硫苏糖醇(DTT)中的种或多种。本发明提供的PCR反应体系和PCR试剂盒与7-deaza-dGTP扩增高片段的方法相比，大大降 |
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本发明属于生物技术领域，具体涉及PCR技术领域，更具体涉及种用于高GC片段扩增的PCR反应体系及试剂盒，所述的PCR反应体系和试剂盒包括：dNTPs、Tris-HCl、MgSO、(NH)SO和PCR助剂。所述的PCR助剂为BeCl、二甲基亚砜(DMSO)、甜菜碱和二硫苏糖醇(DTT)中的种或多种。本发明提供的PCR反应体系和PCR试剂盒与7-deaza-dGTP扩增高片段的方法相比，大大降</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2017</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20170811&DB=EPODOC&CC=CN&NR=107034211A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20170811&DB=EPODOC&CC=CN&NR=107034211A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>PENG CHUNMEI</creatorcontrib><creatorcontrib>MO JINGYAN</creatorcontrib><creatorcontrib>CHEN GUANZHI</creatorcontrib><creatorcontrib>CHEN FENGYING</creatorcontrib><creatorcontrib>WANG FA</creatorcontrib><creatorcontrib>ZHANG XIN</creatorcontrib><creatorcontrib>LI HAIYIN</creatorcontrib><creatorcontrib>LIN MINSHEN</creatorcontrib><creatorcontrib>WANG XING</creatorcontrib><creatorcontrib>YU PEIYU</creatorcontrib><creatorcontrib>YUE XIAOYAN</creatorcontrib><creatorcontrib>XIE LIJUAN</creatorcontrib><creatorcontrib>ZHANG XIAOWEI</creatorcontrib><creatorcontrib>LI JIADAO</creatorcontrib><creatorcontrib>LIN ZHIHAO</creatorcontrib><creatorcontrib>ZHANG JIA</creatorcontrib><creatorcontrib>LIN RUOLIN</creatorcontrib><creatorcontrib>SHI ZHUANGZHUANG</creatorcontrib><creatorcontrib>LUO YUANXIANG</creatorcontrib><creatorcontrib>DENG KEJI</creatorcontrib><title>PCR (polymerase chain reaction) system and kit for high-GC segment amplification</title><description>The invention belongs to the technical field of biology, and particularly relates to the technical field of PCR (polymerase chain reaction), more particular to a PCR (polymerase chain reaction) system and kit for high-GC segment amplification. The PCR system and kit comprises dNTPs, Tris-HCl, MgSO4, (NH4)2SO4 and PCR auxiliary, wherein the PCR auxiliary is one or multiple of BeCl2, dimethyl sulfoxide (DMSO), betaine and dithiothreitol (DTT). Compared with methods for amplifying high segments through 7-deaza-dGTP, the PCR system and kit has the advantage that cost is lowered greatly. Compared with existing high-GC-content amplification methods, the PCR system can amplify DNA segments higher in GC content, can amplify DNA segments higher than 80% in GC content and even can amplify DNA segments up to 90% in GC content.
本发明属于生物技术领域，具体涉及PCR技术领域，更具体涉及种用于高GC片段扩增的PCR反应体系及试剂盒，所述的PCR反应体系和试剂盒包括：dNTPs、Tris-HCl、MgSO、(NH)SO和PCR助剂。所述的PCR助剂为BeCl、二甲基亚砜(DMSO)、甜菜碱和二硫苏糖醇(DTT)中的种或多种。本发明提供的PCR反应体系和PCR试剂盒与7-deaza-dGTP扩增高片段的方法相比，大大降</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2017</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNy7sKwjAUgOEsDqK-w3HTodDYgrMEL5MUcS-HeNIczI0kS99eBB_A6V--fymGQT1gl6KbPWUsBNoiB8iEunIMeyhzqeQBwwveXMHEDJYn21wVFJo8hQrok2PDGr_HWiwMukKbX1diezk_1a2hFEcqCTUFqqO6y_bYdv1BylP3j_kABi02PQ</recordid><startdate>20170811</startdate><enddate>20170811</enddate><creator>PENG CHUNMEI</creator><creator>MO JINGYAN</creator><creator>CHEN GUANZHI</creator><creator>CHEN FENGYING</creator><creator>WANG FA</creator><creator>ZHANG XIN</creator><creator>LI HAIYIN</creator><creator>LIN MINSHEN</creator><creator>WANG XING</creator><creator>YU PEIYU</creator><creator>YUE XIAOYAN</creator><creator>XIE LIJUAN</creator><creator>ZHANG XIAOWEI</creator><creator>LI JIADAO</creator><creator>LIN ZHIHAO</creator><creator>ZHANG JIA</creator><creator>LIN RUOLIN</creator><creator>SHI ZHUANGZHUANG</creator><creator>LUO YUANXIANG</creator><creator>DENG KEJI</creator><scope>EVB</scope></search><sort><creationdate>20170811</creationdate><title>PCR (polymerase chain reaction) system and kit for high-GC segment amplification</title><author>PENG CHUNMEI ; MO JINGYAN ; CHEN GUANZHI ; CHEN FENGYING ; WANG FA ; ZHANG XIN ; LI HAIYIN ; LIN MINSHEN ; WANG XING ; YU PEIYU ; YUE XIAOYAN ; XIE LIJUAN ; ZHANG XIAOWEI ; LI JIADAO ; LIN ZHIHAO ; ZHANG JIA ; LIN RUOLIN ; SHI ZHUANGZHUANG ; LUO YUANXIANG ; DENG KEJI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN107034211A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2017</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>PENG CHUNMEI</creatorcontrib><creatorcontrib>MO JINGYAN</creatorcontrib><creatorcontrib>CHEN GUANZHI</creatorcontrib><creatorcontrib>CHEN FENGYING</creatorcontrib><creatorcontrib>WANG FA</creatorcontrib><creatorcontrib>ZHANG XIN</creatorcontrib><creatorcontrib>LI HAIYIN</creatorcontrib><creatorcontrib>LIN MINSHEN</creatorcontrib><creatorcontrib>WANG XING</creatorcontrib><creatorcontrib>YU PEIYU</creatorcontrib><creatorcontrib>YUE XIAOYAN</creatorcontrib><creatorcontrib>XIE LIJUAN</creatorcontrib><creatorcontrib>ZHANG XIAOWEI</creatorcontrib><creatorcontrib>LI JIADAO</creatorcontrib><creatorcontrib>LIN ZHIHAO</creatorcontrib><creatorcontrib>ZHANG JIA</creatorcontrib><creatorcontrib>LIN RUOLIN</creatorcontrib><creatorcontrib>SHI ZHUANGZHUANG</creatorcontrib><creatorcontrib>LUO YUANXIANG</creatorcontrib><creatorcontrib>DENG KEJI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>PENG CHUNMEI</au><au>MO JINGYAN</au><au>CHEN GUANZHI</au><au>CHEN FENGYING</au><au>WANG FA</au><au>ZHANG XIN</au><au>LI HAIYIN</au><au>LIN MINSHEN</au><au>WANG XING</au><au>YU PEIYU</au><au>YUE XIAOYAN</au><au>XIE LIJUAN</au><au>ZHANG XIAOWEI</au><au>LI JIADAO</au><au>LIN ZHIHAO</au><au>ZHANG JIA</au><au>LIN RUOLIN</au><au>SHI ZHUANGZHUANG</au><au>LUO YUANXIANG</au><au>DENG KEJI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>PCR (polymerase chain reaction) system and kit for high-GC segment amplification</title><date>2017-08-11</date><risdate>2017</risdate><abstract>The invention belongs to the technical field of biology, and particularly relates to the technical field of PCR (polymerase chain reaction), more particular to a PCR (polymerase chain reaction) system and kit for high-GC segment amplification. The PCR system and kit comprises dNTPs, Tris-HCl, MgSO4, (NH4)2SO4 and PCR auxiliary, wherein the PCR auxiliary is one or multiple of BeCl2, dimethyl sulfoxide (DMSO), betaine and dithiothreitol (DTT). Compared with methods for amplifying high segments through 7-deaza-dGTP, the PCR system and kit has the advantage that cost is lowered greatly. Compared with existing high-GC-content amplification methods, the PCR system can amplify DNA segments higher in GC content, can amplify DNA segments higher than 80% in GC content and even can amplify DNA segments up to 90% in GC content.
本发明属于生物技术领域，具体涉及PCR技术领域，更具体涉及种用于高GC片段扩增的PCR反应体系及试剂盒，所述的PCR反应体系和试剂盒包括：dNTPs、Tris-HCl、MgSO、(NH)SO和PCR助剂。所述的PCR助剂为BeCl、二甲基亚砜(DMSO)、甜菜碱和二硫苏糖醇(DTT)中的种或多种。本发明提供的PCR反应体系和PCR试剂盒与7-deaza-dGTP扩增高片段的方法相比，大大降</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | PCR (polymerase chain reaction) system and kit for high-GC segment amplification |
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