PCR (polymerase chain reaction) system and kit for high-GC segment amplification

The invention belongs to the technical field of biology, and particularly relates to the technical field of PCR (polymerase chain reaction), more particular to a PCR (polymerase chain reaction) system and kit for high-GC segment amplification. The PCR system and kit comprises dNTPs, Tris-HCl, MgSO4, (NH4)2SO4 and PCR auxiliary, wherein the PCR auxiliary is one or multiple of BeCl2, dimethyl sulfoxide (DMSO), betaine and dithiothreitol (DTT). Compared with methods for amplifying high segments through 7-deaza-dGTP, the PCR system and kit has the advantage that cost is lowered greatly. Compared with existing high-GC-content amplification methods, the PCR system can amplify DNA segments higher in GC content, can amplify DNA segments higher than 80% in GC content and even can amplify DNA segments up to 90% in GC content. 本发明属于生物技术领域，具体涉及PCR技术领域，更具体涉及种用于高GC片段扩增的PCR反应体系及试剂盒，所述的PCR反应体系和试剂盒包括：dNTPs、Tris-HCl、MgSO、(NH)SO和PCR助剂。所述的PCR助剂为BeCl、二甲基亚砜(DMSO)、甜菜碱和二硫苏糖醇(DTT)中的种或多种。本发明提供的PCR反应体系和PCR试剂盒与7-deaza-dGTP扩增高片段的方法相比，大大降