Culture solution for improving development potential of in-vitro cultured porcine oocytes and its cultivating method

The invention discloses a culture solution for improving maturity quality and development potential of in-vitro cultured porcine oocytes and its cultivating method. The culture solution is prepared from, by weight, 3-7 mu mol/L of forskolin, 3-7 mu mol/L of Roscovitine, 8-12g/L of TCM-199, 1-4g/L of NaHCO3, 0.4-0.7g/L of D-glucose, 0.05-0.15g/L of sodium pyruvate, 0.05-0.1g/L of penicillin sodium, 0.03-0.07g/L of streptomycin sulphate, 1-4g/L of polyving akohol, 0.3-0.7mu g/mL of luteotropin, 0.3-0.7mu g/mL of follicle-stimulating hormone, 8-12ng/mL of epidermal growth factor, 0.3-0.7mmol/L of L- cysteine, and 8-12% of follicular fluid. The culture solution has the advantages of improving cleavage rate, blastaea number and blastaea cell population of porcine oocytes, and effectively improving the development potential of the in-vitro cultured porcine oocytes; so the culture solution has great application prospect. 本发明公开了种提高猪卵母细胞体外成熟质量和发育潜力的培养液及培养方法；所述培养液包括以下组分：毛喉素3～7μmol/L，Roscovitine 3～7μmol/L，TCM-199 8～12 g