Yolk-free human sperm cryoprotectant and preserving method

The invention discloses a yolk-free human sperm cryoprotectant and a preserving method. The yolk-free human sperm cryoprotectant is prepared from, by final concentration during human sperm preservation, 90-110 mmol/L of sodium chloride, 0.5-6 mmol/L of potassium chloride, 0.2-0.5 mmol/L of magnesium...

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Bibliographische Detailangaben
Hauptverfasser: LIN LI, LI FUPING, YUE HUANXUN, JIANG MIN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a yolk-free human sperm cryoprotectant and a preserving method. The yolk-free human sperm cryoprotectant is prepared from, by final concentration during human sperm preservation, 90-110 mmol/L of sodium chloride, 0.5-6 mmol/L of potassium chloride, 0.2-0.5 mmol/L of magnesium sulfate, 2-4 mmol/L of calcium chloride, 0.2-0.5 mmol/L of sodium dihydrogen phosphate, 28-35 mmol/L of sodium bicarbonate, 110-150 mmol/L of glycine, 18-25 mmol/L of 4-xyethyl piperazine ethylsulfonic acid, 5-8 mmol/L of glucose, 30-60 mmol/L of sucrose, 12-15 mmol/L of sodium lactate, 50-100mL/L of glycerin and the balance ultra-pure water. The human sperm cryoprotectant contains no yolk, does not cause allergic reaction, can have the good protecting effect on freezing injuries in the sperm freezing process, has no obvious negative effect on physiological functions of the sperm, and has good forward movement sperm recovery rate after freezing. 本发明公开了种不含卵黄的人精子冷冻保护液及保存方法,其中,精子冷冻保护液包括以下组分且在保存人精子时各组分的终浓度为:氯化钠90~110m