Intravital breeding method of Entomopathogenic nematodes

The invention discloses an intravital breeding method of Entomopathogenic nematodes, and relates to a breeding method of Entomopathogenic nematodes. The method aims to solve the problems that the intravital breeding efficiency of Entomopathogenic nematodes is low and industrialized production cannot be carried out. The method includes the steps that 1, the healthy sixth instar larvae of Prodenia litura of Noctuidae of Lepidoptera are selected as breeding hosts for Entomopathogenic nematodes; 2, the larvae of Prodenia litura are placed in a container, and after the larvae of Prodenia litura are starved, Entomopathogenic nematodes in an infective stage are inoculated to the larvae of Prodenia litura, and infection and reproduction are carried out at room temperature; 3, Entomopathogenic nematodes reproduce in the bodies of the larvae of Prodenia litura, and after the larvae of Prodenia litura die, the dead larvae of Prodenia litura are removed and Entomopathogenic nematodes are collected. According to the intra