Method for site-directed mutation of maize gene by means of CRISPR/Cas9 system

The invention provides a method for site-directed mutation of a maize gene by means of a CRISPR/Cas9 system. An sgRNA sequence based on CRISPR/Cas9 is designed aiming at a target gene in maize, a DNA fragment for encoding the sgRNA sequence is connected to a vector carrying CRISPR/Cas9, and then mai...

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Bibliographische Detailangaben
Hauptverfasser:	ZUO DAN, ZHOU QIAN, HUANG LEI, YANG QIAO, MA CHONGLIE, HU YANLIN, TANG YI, CHENG XIONGYING, WANG WENSHU, TANG TONG, KUANG LE, ZHANG WANGGEN, XU JIETING, ZHOU ZHENGJIAN, LIU TAO
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides a method for site-directed mutation of a maize gene by means of a CRISPR/Cas9 system. An sgRNA sequence based on CRISPR/Cas9 is designed aiming at a target gene in maize, a DNA fragment for encoding the sgRNA sequence is connected to a vector carrying CRISPR/Cas9, and then maize transformation is conducted, so that site-directed mutation of a specific gene in maize is realized. Further, the vector containing CRISPR/Cas9 is transferred to an acceptor material carrying the target gene by means of a genetic transformation method, so as to obtain a site-directed mutation regeneration plant of the target gene. By the adoption of the method, site-directed mutation of a maize genome can be achieved. The method has the advantages that the experimental period is short, and operation is easy. Different target genes can be transformed in a site-directed mode by means of CRISPR/Cas9 systems with different targeting directions, a novel method is provided for improved breeding of maize, and the metho