Rapid nucleic acid extraction method for fluorescent quantitative PCR (polymerase chain reaction) detection

The invention relates to a rapid nucleic acid extraction method for fluorescent quantitative PCR (polymerase chain reaction) detection. The method is applicable to extraction of DNA (deoxyribonucleic acid) of pathogenic microorganisms such as mycoplasmas, Gram negative bacteria and viruses, and the extracted product DNA can be used for fluorescent quantitative PCR detection. Samples of the pathogenic microorganisms such as the mycoplasmas, the Gram negative bacteria and the viruses are washed once by normal saline, solution containing 5% of Chelex-100, 0.5% of NP-40, 0.5% of Triton X-100 and 1mM of EDTA (ethylene diamine tetraacetic acid) is added in precipitation, the pH (potential of hydrogen) of the solution ranges from 11 to 12, the solution is re-suspended, heated for 10 minutes at the temperature of 80-90 DEG C and centrifuged for 5 minutes at the speed of 12000rpm, and supernatant is used for fluorescent quantitative PCR detection. Compared with an existing nucleic acid extraction method, the rapid nuc