Method for detecting feline herpes virus

The invention provides a method for detecting feline herpes virus. The method comprises the following steps: screening to obtain an oligonucleotide primer, and detecting feline herpes virus by adopting a SYBR Green I fluorescent quantitative PCR method. The invention provides a primer pair for detec...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	SHAN HU, ZHANG HONGLIANG, NI BINYAN, ZHANG HUI, SUN QIANG, YANG RUIMEI, SUN JUNCHANG, PAN LIUTING, QIN ZHIHUA, YU PENG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention provides a method for detecting feline herpes virus. The method comprises the following steps: screening to obtain an oligonucleotide primer, and detecting feline herpes virus by adopting a SYBR Green I fluorescent quantitative PCR method. The invention provides a primer pair for detecting feline herpes virus, and the sequences of an upstream primer and a downstream primer are respectively SEQ ID NO:1 and SEQ ID NO:2. The screened primer not only can ensure the accuracy of identified pathogen, but also can not detect congener different strains of pathogen. In addition, the amplified segments have a proper length, thus not only being capable of separating from each other in electrophoresis, but also being incapable of dispersing, and avoiding the situation that small segments are optimally amplified and the amplification of long segments are blocked in multiple PCR; and non-specific amplified products such as a primer dimer and the like. in addition, the high-specificity and high-sensitivity fluo