Prime pair for detecting Stemphylium solani and application of prime pair

The invention discloses a prime pair for detecting Stemphylium solani and application of the prime pair. The prime pair for detecting Stemphylium solani is formed by single-strand DNAs respectively named as Stem-g7F and Stem-g7R; Stem-g7F is the single-strand DNA with a nucleotide sequence represented by SEQ ID No.1 in a sequence table; and Stem-g7R is the single-strand DNA with a nucleotide sequence represented by SEQ ID No.2 in a sequence table. By detecting Stemphylium solani by virtue of the prime pair, the sensitivity is increased by 1000 times and reaches 4.285*10 fg.uL, and accurate quantification can be realized. The prime pair can be used for implementing quantitative determination before the symptomatic appearance of diseases in a preliminary stage and can be applied to field testing of soil-borne fungi and seed-borne fungi. 本发明公开了检测茄匍柄霉菌的引物对及其应用。本发明公开的检测茄匍柄霉菌的引物对由名称分别为Stem-g7F和Stem-g7R的单链DNA组成；Stem-g7F为核苷酸序列是序列表中SEQ ID No.1的单链DNA；Stem-g7R为核苷酸序列是序列表中SEQ ID No.2的单链DNA。利用本发明的引物对检测茄匍柄霉菌的灵敏度提高了1000倍