Kit for endometriosis diagnosis and detection method thereof

The invention provides a kit for endometriosis diagnosis. The kit comprises 1) an EDTA-K2 anticoagulative tube for collecting peripheral blood, 2) a cryopreservation tube for preloading Trizol, 3) a reagent for extracting total RNA in peripheral blood, 4) a reagent for detecting miRNA-141 and U6 gene expression, and 5) a formula of calculating miRNA-141 scores in blood: miRNA-141 score = miRNA-141Ct value - U6Ct value. The kit has high repeatability and stability and no repeated experiment is required. MiRNA-141 and U6 are utilized to amplify a nucleic acid sequence, the diagnosis kit for determining miRNA score in the blood is constructed, the goal of noninvasive diagnosis of endometriosis can be achieved, the damage to patients caused by laparoscope detection is greatly reduced, and the kit is favorable for early treatment of the endometriosis. 本发明提供了种用于子宫内膜异位症诊断的试剂盒，包含：1)收集外周血的EDTA-K2抗凝管；2)预装Trizol的冻存管；3)外周血中总RNA提取试剂；4)检测miRNA-141和U6基因表达的试剂；5)血液中miRNA-141评分的计算公式：miRNA-141分数＝miRNA-141Ct值-U6Ct值。本发明提供的试剂盒有较高的