Mycoplasma gallisepticum culture medium and preparation method thereof

The invention is applicable to the technical field of veterinary biological products, and provides a mycoplasma gallisepticum culture medium. The culture medium comprises a basal culture medium and an auxiliary culture medium. The culture medium mainly comprises glucose, lactoprotein hydrolysate, yeast powder, thallium acetate, sodium chloride, porcine serum, SPF (specific pathogen free) embryo allantoic liquid and SPF embryo amniotic fluid. At the same time, the invention further provides a preparation method of the culture medium.Complicated ingredient substances of the traditional culture medium are simplified; the production cost of an enterprise is lowered; the consumption of the porcine serum is reduced; and an anaphylactic reaction of a chicken caused by the porcine serum is reduced.At the same time, the culture medium can allow viable bacterium titer of mycoplasma gallisepticum to reach above 1023CCU/mL; the viable bacterium titer and the separation sensitivity are much higher than those of the cultur