Tissue culture rapid propagation method for cissus pteroclada hayata serving as characteristic herbal medicine of Guangxi Yao nationality

The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a tissue culture rapid propagation method for cissus pteroclada hayata serving as characteristic herbal medicine of Guangxi Yao nationality. The tissue culture rapid propagation method sequentially comprises the steps of collection of an explant of a cissus pteroclada hayata plant, explant induction culture, multiplication culture, rooting culture and transplanting, wherein an explant induction medium used in the explant induction culture operation is prepared from an MS culture medium, 0.5 to 2.0mg/L 6-BA, 0.2 to 0.5mg/L NAA (Naphthalene Acetic Acid), 0.01 to 0.05mg/L Paclobutrazol, 25 to 30g/L sucrose, 3.5 to 4.0g/L agar and 0.5 to 1.0g/L active carbon, and is 5.4 to 5.8 in pH. A cissus pteroclada hayata stem segment with node is selected as the explant, and is subjected to the steps of induction culture, multiplication culture, rooting culture and transplantation, the induction rate is 88.5 percent or