Double PCR rapid detection kit and method of Portunus trituberculatus Vibrio natriegens

The invention discloses a double PCR rapid detection kit of Portunus trituberculatus Vibrio natriegens. The kit comprises a negative control, a Vibrio natriegens XA1 strain DNA as a positive control, a PCR buffer solution containing Mg, dNTP and DNA polymerase, and a primer pair. The method also discloses a double PCR rapid detection method of Portunus trituberculatus Vibrio natriegens. The method comprises extracting a DNA template, preparing a PCR premix solution, carrying out PCR amplification, after the amplification reaction, carrying out electrophoresis and observing the result through an ultraviolet analyzer, wherein if the bands appear at 308bp and 526bp, the detection result is positive, otherwise, the result is negative. The kit and method have high sensitivity, has the lowest detection limit of 13.2pg of the Vibrio natriegens genome DNA, is easy to operate, is free of specialized technicians, has short whole process time and has low time consumption. 本发明公开了种三疣梭子蟹病原需钠弧菌双重PCR快速检测试剂盒，该试剂盒包括阴性对照、需钠