Rapid screening method of natural alpha-glucosidase inhibitor in rhizoma cyperi

The invention belongs to the technical field of traditional Chinese medicine compound screening, and especially relates to a rapid screening method of natural alpha-glucosidase inhibitor in rhizoma cyperi. The rapid screening method comprises following steps: 1, synthesis of an immobilized enzyme; 2, affinity adsorption and desorption of the immobilized enzyme; 3, determination of the structure of rhizoma cyperi AGHI; and 4, verification of the activity of rhizoma cyperi AGHI. The advantages of the rapid screening method are that: complex and time-consumed separation process is not needed, and an active inhibitor monomer can be separated from a natural product mixture via screening in a short time period (a few hours). 本发明属于中药化合物筛选技术领域，尤其涉及种香附药材中天然α-葡萄糖苷酶抑制剂的快速筛选方法。具体包括以下步骤：步骤1、固定化酶的合成；步骤2、固定化酶的亲和吸附与解吸；步骤3、香附AGHI的结构确定：步骤4、香附AGHI的活性验证；该方法的优点在于无需复杂耗时的分离工作，可在较短时间内（数小时）筛选出天然产物混合物中的活性抑制剂单体。