Preparation method of antiserum for CMV (cucumber mosaic virus) infecting Zingiber officinale Rosc

The invention discloses a preparation method and an application of antiserum for CMV (cucumber mosaic virus) infecting Zingiber officinale Rosc. The preparation method comprises followings steps: extracting and purifying a coarse virus, preparing gel of CMV coat protein, adding normal saline to the gel containing coat protein, grinding the mixture to be fine, and performing immune injection on a mouse to obtain the antiserum. Electrophoretic separation coat protein is prepared from different dosages of CMV particles and 5%-20% of SDS-PAGE gradient gel, the mouse is immunized with the coat protein and polyacrylamide gel, the high-titer antiserum is obtained, and the technology is practical and reliable accordingly. When the antiserum prepared with the method is applied to detection of the CMV infecting Zingiber officinale Rosc, the CMV infecting Zingiber officinale Rosc can be detected rapidly, and detection of the virus is greatly facilitated. 本发明公开了种制备侵染生姜黄瓜花叶病毒抗血清的制备方法及其应用，包括以下步骤：粗病毒的提取和纯化、制备黄瓜花叶病毒外壳蛋白的凝胶、将