Method for preparing pork powder standard substance with clenbuterol

The invention discloses a method for preparing a pork powder standard substance with clenbuterol. The method comprises the following steps: performing simulated feeding so as to obtain a pork raw material polluted by clenbuterol, crushing a sample, directly mixing with water in a proper ratio, preparing freeze-dried pork by using a freeze-drying method directly without homogenation, and implementing steps of grinding, screening, uniform mixing, radiation and the like, thereby obtaining freeze-dried pork powder in good uniformity. Value confirmation, uniformity inspection and stability monitoring of standard substances are implemented by using a liquid chromatogram isotope dilution mass spectrometry (LC-ID-MS/MS) method for verifying accuracy through international comparison. The pork powder standard substance with clenbuterol, which is prepared by using the method, is good in uniformity and stability, and can be applied to analysis method evaluation for residue detection on clenbuterol in pork, quality contro