Clostridium ghonii specific PCR detection primers and method

The invention relates to clostridium ghonii specific PCR detection primers and a method. Nucleotide sequence of a forward primer of the pair of clostridium ghonii specific PCR detection primers is shown as SEQ ID NO.1, and nucleotide sequence of a reverse primer is shown as SEQ ID NO.2. According to the invention, it is discovered for the first time that an encoding gene Trx of thioredoxin obeys to a highly conserved law which is different from other thioredoxin gene sequences, which keep a close genetic relationship, such as clostridium novyi, clostridium beijerinckii, clostridium sporogene and the like; and on the basis of the finding, the clostridium ghonii specific PCR detection primers are designed, so that technical difficulties in clostridium ghonii in-vivo distribution and clinical detection by virtue of a PCR technology are solved. 本发明涉及种戈氏梭菌特异性PCR检测引物及方法。戈氏梭菌特异性PCR检测引物，该引物为对，正向引物核苷酸序列如SEQ ID NO.1所示，反向引物核苷酸序列如SEQ ID NO.2所示。本发明首次公开了戈氏梭菌硫氧还蛋白的编码基因Trx不同于Clostridium Novyi、Clostridium beijerinckii、Clostri