Specific nanometer antibody of PI3K, and clinical applications thereof

The invention discloses a nanometer antibody VHH chain of PI3K. The nanometer antibody VHH chain of PI3K comprises a framework region FR and a complementary determining region CDR; the amino acid sequence of the framework region FR selected from the FR amino acid sequences disclosed in the invention, and the amino acid sequence of the complementary determining region CDR are disclosed. The invention also discloses a PI3K nanometer antibody, a DNA molecule used for encoding the nanometer antibody VHH chain of PI3K or the nanometer antibody of PI3K, a host cell used for expressing the nanometer antibody of PI3K, and applications of the nanometer antibody of PI3K in detecting PI3K. High efficiency expression of the nanometer antibody in Escherichia coli can be realized via applications of the nanometer antibody gene sequence and the host cell, and applications in the development of PI3K detection reagent and biological pharmacy are realized. 本发明公开了种PI3K的纳米抗体的VHH链，包括框架区FR和互补决定区CDR，公开了框架区FR选自下组的FR的氨基酸序列和互补决定区CDR氨基