Preparation method of frozen bovine semen diluting liquid

The invention discloses a preparation method of frozen bovine semen diluting liquid. The preparation method comprises the following steps: weighing 5g to 8g of fructose and 19.8g to 26.6g of sodium citrate; adding 800mL of distilled water to stir and filtering a solution with filter paper into a conical flask or a saline solution bottle; putting into a water-bath pot at 75 DEG C to 80 DEG C and disinfecting for 30min; cooling to room temperature and adding 180mL to 220mL of fresh yolk; adding 0.4 to 0.5 million IU units of penicillin and 0.4 to 0.5 million IU units of streptomycin, wherein a solution prepared according to the ratio is named as a first solution; adding 430mL of the first solution into 65mL to 75mL of glycerol; after stirring, putting a mixture into a refrigerator at 3 DEG C to 5 DEG C to obtain a second solution; then diluting semen, freezing and packaging. By adopting the preparation method, a fresh semen diluting effect and the live rate of sperm of excellent breeding bulls are improved, and