Primer and method for amplifying CDS sequence of bird IFNA gene

The invention discloses a primer and a method for amplifying a CDS sequence of a bird IFNA gene. The primer comprises a DNIFNA-F of which the serial number is SEQ ID NO.1 and a DNIFNA-R of which the serial number is SEQ ID NO.2. The method comprises the steps of (1) extracting a total genome DNA of...

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Hauptverfasser: WANG YING, YI SHUANGLONG, DING HENGWU, JIANG LAN, WANG HUIMEI, CHEN RENRUI, KAN XIANZHAO, ZHANG QIN, WU XUAN, WAN FENGYING, WANG QINGQING
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a primer and a method for amplifying a CDS sequence of a bird IFNA gene. The primer comprises a DNIFNA-F of which the serial number is SEQ ID NO.1 and a DNIFNA-R of which the serial number is SEQ ID NO.2. The method comprises the steps of (1) extracting a total genome DNA of a to-be-extracted sample; (2) carrying out PRC amplification on the total genome DNA extracted in the step (1) by adopting the primer; and (3) carrying out agarose gel electrophoresis detection on the product amplified in the step (3). One-time PCR amplification is carried out on a to-be-detected sample by designing a pair of specific PCR primers, and whether a stripes exist or not is detected through agarose gel electrophoresis, so that the judgment and amplification effect is displayed; the used template can be extracted from animal muscle tissue; and the sampling difficult is greatly reduced. 本发明公开了种用于扩增鸟类IFNA基因CDS序列的引物及其方法,所述引物包括序列号为SEQ ID NO.1的DNIFNA-F和序列号为SEQ ID NO.2的DNIFNA-R。所述方法包括:(1)提取待测样品的总基因组DNA;(2)采用所述引