Antibody testing method for potency of CFS (classical swine fever) genetic engineering subunit vaccine

The invention provides a method for testing potency of a CFS (classical swine fever) virus genetic engineering subunit vaccine. The method comprises steps as follows: healthy susceptible rabbits are taken as test objects and divided into a control group and immunization groups; the CFS virus genetic...

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Hauptverfasser: LIU LEI, WANG LIPING, TAO XIAOSHAN, ZHAO TONGYUAN, ZOU MIN, SUN JIAN, ZHANG HENG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a method for testing potency of a CFS (classical swine fever) virus genetic engineering subunit vaccine. The method comprises steps as follows: healthy susceptible rabbits are taken as test objects and divided into a control group and immunization groups; the CFS virus genetic engineering subunit vaccine is injected into rabbits of the immunization groups while no substances are injected to rabbits of the control group; after 21 days of vaccine injection, the rabbits of the immunization groups and the control group are subjected to blood sampling from hearts, heart blood is centrifuged, then liquid supernatant is taken, a CFS ELSIA (enzyme linked immunosorbent assay) antibody is detected, and when at least 4/5 of the antibody is positive, the test for the CFS virus genetic engineering subunit vaccine is qualified. The method for testing the potency of the CFS virus genetic engineering subunit vaccine can replace a potency testing method adopting swine for immunity challenge; the test an