Nucleic acid composition for detecting HPV (Human Papilloma Virus) types as well as application and kit thereof

The invention discloses a nucleic acid composition for detecting HPV (Human Papilloma Virus) types as well as application and a kit thereof and belongs to the technical field of gene detection. The nucleic acid composition for detecting HPV types comprises a first nucleic acid composition used for detecting HPV16 subtypes and a second nucleic acid composition used for detecting HPV18 subtypes, wherein the first nucleic acid composition comprises first primer pair expressed as SEQ ID NO.1-2 and a first capture probe expressed as SEQ ID NO. 3; the second nucleic acid composition comprises a second primer pair expressed as SEQ ID NO.4-5 and a second capture probe expressed as SEQ ID NO. 6. The nucleic acid composition takes PCR and EFIRM technologies as base, is capable of detecting classes of the HPV subtypes in the samples with high sensitivity and high specificity, has the characteristics of short detection time and low detection cost, and can be applied to a large amount of clinical detection and large-scale