Primers for real-time fluorescence PCR specific detection of licorice roots and detection method

The invention discloses high-specificity and high-sensitivity primers for real-time fluorescence PCR specific detection of licorice roots. The DNA sequences of the primers are shown in SEQ ID NO.1 and SEQ ID NO.2 respectively. A reaction system for detection includes 12.5 [mu]L of SYBR-Premix Ex Taq with two times concentration, 0.25 [mu]L of upstream primer with the concentration of 10 [mu]mol/L, 0.25 [mu]L of downstream primer with the concentration of 10 [mu]mol/L, 1 [mu]L of DNA template of a sample to be detected with concentration lower than 50 ng, and 11[mu]L of double distilled water. Reaction parameters for degeneration are 95 DEG C and 30 s, reaction parameters for amplification are 95 DEG C, 5 s, 60 DEG C and 30 s, and the cycle index is 30. The sample is identified as licorice roots if a specific amplification curve is generated within 30 cycles. 本发明公开种特异性好、灵敏度高的实时荧光PCR特异性检测甘草的引物，所述引物的DNA序列分别如SEQ ID NO.1和SEQ ID NO.2所示。检测的反应体系为：2X浓度的SYBRPremix Ex Taq 12.5 μL、浓度为10μmol/L的上游引物0.25 μL、浓度为10μmol/L的下游引物