Kit and application thereof

The invention relates to a kit and application thereof. Particularly, the kit comprises a cell nucleus separating solution, wherein the cell nucleus separating solution is prepared from 20 to 50 mmol/L of Tris, 0.01 to 0.2 percent of Triton X-100, 1 to 10 mmol/L of EDTA, 5 to 50 mmol/L of MgCl2, and...

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Bibliographische Detailangaben
Hauptverfasser:	LIU YUFANG, ZOU AILAN, SUN KEFEI, LI XIAOCHEN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a kit and application thereof. Particularly, the kit comprises a cell nucleus separating solution, wherein the cell nucleus separating solution is prepared from 20 to 50 mmol/L of Tris, 0.01 to 0.2 percent of Triton X-100, 1 to 10 mmol/L of EDTA, 5 to 50 mmol/L of MgCl2, and 5 to 50 mmol/L of KCl. Therefore, the kit can be used for effectively pretreating blood, so as to obtain cell nucleus sediments for subsequent treatment. 本发明涉及试剂盒及其应用，具体地，所述试剂盒包含：细胞核分离液，其中，所述细胞核分离液包含：20-50mmol/L Tris；0.01-0.2％Triton X-100；1-10mmol/L EDTA；5-50mmol/L MgCl；以及5-50mmol/L KCl。由此，利用所述试剂盒可以有效地对血液进行前处理，从而获得细胞核沉淀，便于后续处理。