Ppmarl transposase C2961 mutant with high catalytic activity and application thereof

The invention discloses a Ppmarl transposase C2961 mutant with high catalytic activity. An amino acid sequence of the Ppmarl transposase C2961 mutant is shown in SEQ ID NO.1. A nucleotide sequence for encoding a gene of the Ppmarl transposase C2961 mutant is shown in SEQ ID NO.2. Cysteine on 296 site of the wild Ppmarl transposase is mutated into isoleucine. The Ppmarl transposase C2961 mutant has the advantage that the transposition activity of catalytic transposon is equal to 2.67 times of the activity of the wild transposase, so as to lay a foundation for utilizing the MLE (Mariner-like element) transposon to develop gene tags, and provide a new tool for studying the gene functions for the large-scale separating and marking of genes in post-genome era. 本发明公开了种具有高催化活性的Ppmar1转座酶C296I突变体，所述的Ppmar1转座酶C296I突变体的氨基酸序列如SEQ ID NO.1所示。编码所述Ppmar1转座酶C296I突变体的基因的核苷酸序列如SEQ ID NO.2所示。是将野生型Ppmar1转座酶296位置上的半胱氨酸突变为异亮氨酸。该Ppmar1转座酶C296I突变体催化转座子转座的活性是野生型转座酶的活性的2.67倍，为利用MLE转座子开发基因标签奠定了基础，为后基因组时代大规模分离和标记基因，研究基因的功能提供了新工具。