Mass spectrum sensitivity enhanced isothiocyanate labeling reagent, and synthesis method and applications thereof

The invention belongs to the technical field of organic micromolecular peptide markers, and specifically relates to a mass spectrum sensitivity enhanced isothiocyanate labeling reagent, and a synthesis method and applications thereof. The labeling reagent is 1-(3-isothiocyanopropyl)-4-phenyl piperazine. The synthesis method comprises the following steps: reacting 1,3-dibromopropane with KOH and N-phenyl piperazine to generate an intermediate namely 1-(3-bromopropyl)-phenyl piperazine; and then adding potassium thiocyanate, polyethylene glycol, and dichloromethane into the intermediate to carry out reactions to generate the target product namely 1-(3-isothiocyanopropyl)-4-phenyl piperazine. The provided isothiocyanate labeling reagent can rapidly, accurately, and sensitively label peptide compounds, is especially suitable for the separation and detection of trace micromolecular peptide compounds in a complicated system, and has a wide application prospect. 本发明属于有机小分子肽类标记物技术领域，具体涉及种质谱增敏的异硫氰酸酯类标记试剂及其合成方法和应用。所述标记