Leiothrix lutea karyogene primers and sequencing method and application thereof

The invention discloses leiothrix lutea karyogene primers and a sequencing method and an application thereof. The primers comprise a first primer pair, a second primer pair and a third primer pair; the first primer pair comprises RAG-F1-17 having a sequence number of SEQ ID NO.1 and RAG-R1-533 having a sequence number of SEQ ID NO.2; the second primer pair comprises RAG-F2-996 having a sequence number of SEQ ID NO.3 and RAG-R2-2024 having a sequence number of SEQ ID NO.4; the third primer pair comprises RAG-F3-1945 having a sequence number of SEQ ID NO.5 and RAG-R3-2779 having a sequence number of SEQ ID NO.6. The obtained primers have quite good amplification function and are used for amplification of a leiothrix lutea karyogene rag1, finally, bright strips can be obtained, and follow-up study of large-scale use of the primers for amplification is facilitated. 本发明公开了种红嘴相思鸟核基因引物及其测序方法和应用，所述引物包括第引物对、第二引物对和第三引物对，所述第引物对包括序列号为SEQ ID NO.1的RAG-F1-17和序列号为SEQ ID NO.2的RAG-R1-533；所述第二引物对包括序列号为SEQ ID NO.3的RAG-F2-996和序列号