Preparation method of SBA-15 immobilized angiotensin converting enzyme

The invention provides a preparation method of an SBA-15 immobilized angiotensin converting enzyme (ACE). A mesoporous molecular sieve SBA-15 chelated with zinc ions is used as a carrier and an angiotensin converting enzyme is used as an immobilization object, and the carrier and the immobilization object are subjected to adsorption and bonding effects to obtain the SBA-15 immobilized angiotensin converting enzyme. In a product obtained by the method, the ACE is immobilized in pore channels of the zinc ion chelated mesoporous molecular sieve SBA-15 and an outer surface of the molecular sieve. According to the preparation method, an immobilization process for preparing the Zn-SBA-15 immobilized ACE is simple and the stability of the product is good; and compared with a free enzyme, the prepared Zn-SBA-15 immobilized ACE has better temperature and pH (Potential of Hydrogen) stability. 本发明提供了种SBA-15固定化血管紧张素转化酶的制备方法，利用螯合锌离子的介孔分子筛SBA-15为载体，以血管紧张素转化酶为固定对象，通过吸附与键和作用，得到SBA-15固定化血管紧张素转化酶。上述方法得到的产品中，ACE在螯合锌离子的介孔分子筛SBA-