METHOD FOR FABRICATING ELECTROCHEMICAL SENSING TEST PIECE

A method for fabricating an electrochemical sensing test piece comprises steps: forming an electrode layer on a substrate; etching the electrode layer to reduce the area of the electrode layer to be smaller than the area of the substrate, wherein the electrode layer has a test zone and a reading zone neighboring the test zone; forming an insulation member surrounding the test zone and covering the perimeter of the test zone; forming an enzyme layer on the test zone; and forming an insulation layer on the enzyme layer and the periphery of the reading zone and fabricating the insulation layer to have an opening revealing a portion of the enzyme layer. The insulation member fixes the effective reaction area of the tested material and increases measurement accuracy. 本发明公开种电化学感测试片的制作方法，为先将电极层形成于基板上，再对该电极层进行蚀刻，使该电极层的面积小于该基板的面积，且该电极层具有检测部以及相邻于该检测部的读取部，而后，形成绝缘部于该电极层的该检测部的周围，且该绝缘部覆盖住该检测部的周缘，接着，形成酵素层于该检测部之上，最后，形成绝缘层于该酵素层之上以及该读取部的周围，并使该绝缘层具有露出部分该酵素层的开口，借由该绝缘部的设置，可以固定与待检测物反应的有效反应面积，而可提高量测的准确性。