Primer pair for detecting salmon alpha viruses, SYBR Green I fluorescent quantitative PCR (Polymerase Chain Reaction) kit and application thereof

The invention discloses a primer pair for detecting salmon alpha viruses, an SYBR Green I fluorescent quantitative PCR (Polymerase Chain Reaction) kit and application thereof. The invention provides the real-time fluorescent quantitative PCR primer pair for detecting the salmon alpha viruses for the first time; and the sequence is shown as SEQ ID NO.2 and SEQ ID NO.3. The invention further provides the SYBR Green I fluorescent quantitative PCR kit containing the primer pair and a method for detecting the salmon alpha viruses. A research shows that the primer or the kit, disclosed by the invention, is used for detecting the salmon alpha viruses, has the advantages of simplicity, rapidness, accuracy, high sensitivity and good specificity and the like, and can be used for detecting six gene types of the salmon alpha viruses. The invention provides an effective technical means for detecting the salmon alpha viruses. 本发明公开了种检测鲑鱼甲病毒的引物对、SYBR GreenⅠ荧光定量PCR试剂盒及其应用。本发明首先提供了用于检测鲑鱼甲病毒的实时荧光定量PCR引物对，其序列分别如SEQ ID NO.2，SEQ